Coding

Part:BBa_K5048047:Design

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-23)


Neae-FLAG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1244


Design Notes

This is a surface display system that we used to present our adhesins LAP and HSP60 on the outer membrane of E. coli. Condon optimized for better expression in our chassis E.coli. Cooperated with Neae and LAP, we expected to achieve cell-cell adhesion and cell-intestine adhesion in the small intestine(Fig 1).


Fig 1 The expected effect for the Adhesion module, which adheres to the engineering bacteria and intestine


Source

Original sequence found in the paper:Glass DS, Riedel-Kruse IH. A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Cell. 2018 Jul 26;174(3):649-658.e16. doi: 10.1016/j.cell.2018.06.041. Epub 2018 Jul 19. PMID: 30033369. UniProt：H3JUY6. We used the N-terminal domain of the protein.


References

Glass DS, Riedel-Kruse IH. A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Cell. 2018 Jul 26;174(3):649-658.e16. doi: 10.1016/j.cell.2018.06.041. Epub 2018 Jul 19. PMID: 30033369.